| Chisso
America, Inc. |
 |
555 Theodore Fremd
Avenue, |
| |
Suite C-206 |
| |
Rye, NY 10580 |
| |
TEL: 914-921-5400 |
| |
FAX: 914-921-8822 |
 |
info@chissony.com |
|
|
|
|
|
Cellufine - Gel Filtration - GCL-2000 |
|
Gel Filtration Chromatography Media Cellufine |
TM |
 |
GCL-2000 |
|
Gel Filtration, or size exclusion chromatography, has proven to be a simple and effective purification method for proteins and other biomolecules. However, the traditionally slow flow rates and limiting resolving power of most gel filtration media have restricted its usefulness. These problems, inherent to the packings, existed not only at the laboratory preparative scale but especially at process scale. The limited throughput of gel filtration columns has often created a significant process bottleneck.
Cellufine GCL media overcome these limitations. The mechanical strength of the spherical cellulose matrix allows high flow rates, even in large industrial columns.
|
Features |
• Rigid spherical particles
• Outstanding mechanical strength
• Hydrophilic
• High pore volume
• Broad selection of fractionation range
• Supplied preswollen
• High chemical resistance
• Autoclavable at 121 °C for 30 minutes
|
Benefits |
• High flow rate compatibility even in large diameter process columns
• May be pumped or stirred without fines generation
• Low non-specific adsorption and high recovery
• Good capacity
• Optimize selectivity to required molecular weight range
• No time-consuming swelling procedures required
• Usable with all common salts, detergents, chaotropic agents, organic solvents
• Depyrogenation
• Sterilizable
• Regulatory Support
|
Characteristics |
| Matrix |
Cross linked cellulose |
| Particle Shape |
Spherical |
| Particle Size |
GCL-2000, 53 – 125 µm |
| Chemical Resistance |
Media can be used with most salts, detergents, solvents, acids and bases, including 8M urea, 6M guanidine / HCL, 0.1M HCL and 0.5M NaOH |
| Mechanical Resistance |
Media will withstand peristaltic pumping and extended magnetic stirring |
| Schrinkage / Swelling
|
Shrinkage or swelling < 3 % with changes in pH or ionic strength. Shrinkage or swelling with solvents varies with solvent and media
|
| Autoclavable |
121 °C for 30 minutes |
| Supplied |
Suspension in 20 % ethanol |
|
Range of Fractionalization |
This media offer an extraordinarily broad selection of fractionation ranges: from the unique Cellufine GCL-2000 for very high molecular weight protein complexes. Table 1 shows the exclusion limit for the media. |
|
Cellufine Packing |
Exclusion Limit (Daltons) |
| PEG |
Proteins |
Polysaccharides |
| GCL-2000 |
200,000 |
3,000,000 |
— |
|
|
Selectivity |
| Protein |
Daltons |
Kav |
Thyroglobulin
Ferritin
Catalase
lgG
Aldolase
Alcohol-Dehydrogenase
Bovine Serum Albumun
Ovalbumin
ß-Lactoglobulin
Chymotrypsinogen A
Myoglobin
Ribonuclease
Cytochrome C
Insulin B-Chain
Bacitracin
(Glycine)4
Glycine |
660,000
440,000
210,000
160,000
58,000
150,000
67,000
46,000
42,000
25,000
17,000
14,000
12,500
3,500
1,400
246
75 |
0.18
0.25
0.33
0.33
0.35
0.35
0.39
0.47
0.51
0.60
0.60
0.65
0.67
—
0.90
—
— |
|
|
Pressure Flow Curves |
|
Protein |
% Recovery |
| Catalase |
100 |
| lgG |
100 |
| Aldolase |
100 |
| Serum Albumin |
100 |
| Lactoglobulin |
100 |
| Ribonuclease |
100 |
| Cytochrome C |
100 |
| Lysozyme |
100 |
| Chymotrypsinogen A |
99 |
| Myoglobin |
97 |
|
Ordering Information |
| Description |
Quantity |
Catalogue No |
Cellufine
GCL-2000 |
100 ml
0.5 L
5 L
10 L |
6720 003 27
19791
19792
6720 003 35 |
|
Contact us |
| Americas |
Ivars Bemberis
Director, Cellufine Chromatography
Mobile : (804) 683-7867
Fax : (804) 739-7422
E-mail : ivars10@comcast.net
|
|
| Europe, Asia, and others |
Emil Zavadsky, Ph.D.
Director, Cellufine Chromatography
Mobile : +41/79-206 81 60
Fax : +41/24-499 30 49
E-mail : emil.zavadsky@bluewin.ch
|
|
|
Ordering Information |
Chisso Corporation
Fine Chemicals Sales Dept.
2-1, Otemachi 2-Chome, Chiyoda-ku,
Tokyo 100-8105 Japan
Tel : +81-3-3243-6150
Fax : +81-3-3243-6219
E-mail : cellufine@chisso.co.jp
|
|
Chisso America Incorporated
555 Theodore Fremd Ave.,
Suite C-206,
Rye, New York 10580 U.S.A.
Tel : 914-921-5400
Fax : 914-921-8822
E-mail : Cellufine@chissony.com
|
|
|
|
|
